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Reference Database
Filter is OFF. Record 1 of 1182
(Journal/book in red = download/link available.)
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EndNote#:
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537
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Reference Type:
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Journal Article
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Author:
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Abbott, G.W.;Bloemendal, M.;Van Stokkum, I.H.;Mercer, E.A.;Miller, R.T.;Sewing, S.;Wolters, M.;Pongs, O.;Srai, S.K.
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Article/
Book Sect.:
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Secondary structure, stability and tetramerisation of recombinant K(V)1.1 potassium channel cytoplasmic N-terminal fragment
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Journal/
Book:
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Biochimica et Biophysica Acta
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Volume:
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1341
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Number:
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1
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Pages:
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71-8
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Label:
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Abstract:
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The recombinant N-terminal fragment (amino acids 14-162) of a tetrameric voltage-gated potassium channel (K(V)1.1) has been studied using spectroscopic techniques. Evidence is presented that it forms a tetramer in aqueous solution, whereas when solubilised in 1% Triton X-100 it remains monomeric. The secondary structure content of both monomeric and tetrameric K(V)1.1 N-terminal fragment has been estimated from FTIR and CD spectroscopy to be 20-25% alpha-helix, 20-25% beta-sheet, 20% turns and 30-40% random coil. Solubilisation of the protein in detergent is shown by hydrogen-deuterium exchange analysis to alter tertiary structure rather than secondary structure and this may be the determining factor in tetramerisation ability. Using molecular modelling we propose a supersecondary structure consisting of two structural domains.
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PDF Paper or WWW Link: (See our software notes page for help with the PDF files.)
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